LOCUS pIEx-10 3801 bp DNA circular 1-SEP-2005
DEFINITION pIEx-10 vector sequence.
SOURCE
ORGANISM
COMMENT VNTNAME|pIEx-10|
COMMENT VNTREPLTYPE|Plasmid
COMMENT VNTEXTCHREPL|Bacteria
COMMENT VNTEXTCHREPL|Animal/Other Eukaryotic
FEATURES Location/Qualifiers
5'UTR 1034..1082
/vntifkey="52"
/label=IE1\5'-UTR
promoter 487..1033
/vntifkey="29"
/label=IE1\Promoter
enhancer 1..482
/vntifkey="9"
/label=hr5
CDS 1760..2617
/vntifkey="4"
/label=Ap
rep_origin 3378..3378
/vntifkey="33"
/label=Ori
polyA_signal 1495..1500
/vntifkey="25"
/label=PolyA\signal
misc_feature 1463..1466
/vntifkey="21"
/label=3x\-BseR\I\TCCT\to\CTTC
terminator 1318..1625
/vntifkey="43"
/label=IE1\Terminator
misc_feature 1246..1260
/vntifkey="21"
/label=C-Term\Ek/LIC\overhang
protein_bind 1186..1200
/vntifkey="31"
/label=Ek\site
misc_binding 1270..1299
/vntifkey="20"
/label=10x\His-Tag
primer 1180..1263
/vntifkey="27"
/label=S-EkLIC\MCS
primer complement(1186..1269)
/vntifkey="27"
/label=AS-EkLIC\MCS
primer 1264..1312
/vntifkey="27"
/label=S-CTerm\10xHis
source 1084..1149
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57 BL/6"
/db_xref="taxon:10090"
/vntifkey="98"
sig_peptide 1084..1140
/vntifkey="94"
/label=Mouse\IgM\signal\seq
mat_peptide join(1141..1230,1435..1500)
/product="idiotypic anti-NP IgM heavy chain V-D-J"
/vntifkey="84"
/label=Mat_Peptide_1
misc_feature 1141..1149
/vntifkey="21"
/label=Misc_Feature_1
/note="variable region (aa 1-98)"
primer 1150..1172
/vntifkey="27"
/label=S-SigSeq\Strep\BstBI
primer 1083..1149
/vntifkey="27"
/label=S-mIgM\sigseq\NcoI
primer complement(1087..1154)
/vntifkey="27"
/label=AS-mIgM\sigseq\NcoI
protein_bind 1156..1179
/vntifkey="31"
/label=Strep-Tag\II
BASE COUNT 1003 a 873 c 896 g 1029 t
ORIGIN
1 cgcgtaaaac acaatcaagt atgagtcata agctgatgtc atgttttgca cacggctcat
61 aaccgaactg gctttacgag tagaattcta cttgtaacgc acgatcagtg gatgatgtca
121 tttgtttttc aaatcgagat gatgtcatgt tttgcacacg gctcataaac tcgctttacg
181 agtagaattc tacgtgtaac gcacgatcga ttgatgagtc atttgttttg caatatgata
241 tcatacaata tgactcattt gtttttcaaa accgaacttg atttacgggt agaattctac
301 ttgtaaagca caatcaaaaa gatgatgtca tttgtttttc aaaactgaac tcgctttacg
361 agtagaattc tacgtgtaaa acacaatcaa gaaatgatgt catttgttat aaaaataaaa
421 gctgatgtca tgttttgcac atggctcata actaaactcg ctttacgggt agaattctac
481 gcgcgtcgat gtctttgtga tgcgcgcgac atttttgtag gttattgata aaatgaacgg
541 atacgttgcc cgacattatc attaaatcct tggcgtagaa tttgtcgggt ccattgtccg
601 tgtgcgctag catgcccgta acggacctcg tacttttggc ttcaaaggtt ttgcgcacag
661 acaaaatgtg ccacacttgc agctctgcat gtgtgcgcgt taccacaaat cccaacggcg
721 cagtgtactt gttgtatgca aataaatctc gataaaggcg cggcgcgcga atgcagctga
781 tcacgtacgt ccctcgtgtt ccgttcaagg acggtgttat cgacctcaga ttaatgttta
841 tcggccgact gttttcgtat ccgctcacca aacgcgtttt tgcattaaca ttgtatgtcg
901 gcggatgttc tatatctaat ttgaataaat aaacgataac cgcgttggtt ttagagggca
961 taataaaaga aatattgtta tcgtgttcgc cattagggca gtataaattg acgttcatgt
1021 tggatattgt ttcagttgca agttgacact ggcggcgaca agatcgtgaa caaccaagtg
1081 accatgaaat tcagctgggt catgttcttc ctgatggcag tggttacagg ggtcaattca
1141 gaggttcagg caagctggag ccacccgcag ttcgaaaagg gtgcagatga cgacgacaag
1201 gtaccggatc cgacgtcggt cgacaagctt gcggccgcag gagctccggg cttctcctca
1261 atttccgctc atcaccacca tcatcaccat caccaccact aagtgattaa cctcaggtta
1321 tacatatatt ttgaatttaa ttaattatac atatatttta tattattttt gtcttttatt
1381 atcgaggggc cgttgttggt gtggggtttt gcatagaaat aacaatggga gttggcgacg
1441 ttgctgcgcc aacaccacct cccttccctc ctttcatcat gtatctgtag ataaaataaa
1501 atattaaacc taaaaacaag accgcgccta tcaacaaaat gataggcatt aacttgccgc
1561 tgacgctgtc actaacgttg gacgatttgc cgactaaacc ttcatcgccc agtaaccaat
1621 ctagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
1681 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
1741 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
1801 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
1861 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
1921 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
1981 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
2041 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
2101 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
2161 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
2221 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
2281 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
2341 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
2401 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
2461 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
2521 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
2581 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
2641 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
2701 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
2761 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
2821 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
2881 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
2941 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
3001 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
3061 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
3121 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
3181 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
3241 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
3301 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
3361 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
3421 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
3481 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
3541 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
3601 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
3661 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
3721 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
3781 ccatgattac gaattcccgg g
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