LOCUS pIEx-8 3738 bp DNA circular 1-SEP-2005
DEFINITION pIEx-8 vector sequence.
SOURCE
ORGANISM
COMMENT VNTNAME|pIEx-8|
COMMENT VNTREPLTYPE|Plasmid
COMMENT VNTEXTCHREPL|Bacteria
FEATURES Location/Qualifiers
5'UTR 1034..1082
/vntifkey="52"
/label=IE1\5'-UTR
promoter 487..1033
/vntifkey="29"
/label=IE1\Promoter
enhancer 1..482
/vntifkey="9"
/label=hr5
CDS 1697..2554
/vntifkey="4"
/label=Ap
rep_origin 3315..3315
/vntifkey="33"
/label=Ori
polyA_signal 1432..1437
/vntifkey="25"
/label=PolyA\signal
misc_feature 1400..1403
/vntifkey="21"
/label=3x\-BseR\I\TCCT\to\CTTC
terminator 1255..1562
/vntifkey="43"
/label=IE1\Terminator
misc_feature 1183..1197
/vntifkey="21"
/label=C-Term\Ek/LIC\overhang
protein_bind 1123..1137
/vntifkey="31"
/label=Ek\site
protein_bind 1093..1116
/vntifkey="31"
/label=Strep-Tag\II
misc_binding 1207..1236
/vntifkey="20"
/label=10x\His-Tag
primer 1117..1200
/vntifkey="27"
/label=S-EkLIC\MCS
primer complement(1123..1206)
/vntifkey="27"
/label=AS-EkLIC\MCS
primer 1083..1116
/vntifkey="27"
/label=S-NTerm\Strep
primer complement(1087..1122)
/vntifkey="27"
/label=AS-NTerm\Strep
primer 1201..1249
/vntifkey="27"
/label=S-CTerm\10xHis
BASE COUNT 989 a 862 c 877 g 1010 t
ORIGIN
1 cgcgtaaaac acaatcaagt atgagtcata agctgatgtc atgttttgca cacggctcat
61 aaccgaactg gctttacgag tagaattcta cttgtaacgc acgatcagtg gatgatgtca
121 tttgtttttc aaatcgagat gatgtcatgt tttgcacacg gctcataaac tcgctttacg
181 agtagaattc tacgtgtaac gcacgatcga ttgatgagtc atttgttttg caatatgata
241 tcatacaata tgactcattt gtttttcaaa accgaacttg atttacgggt agaattctac
301 ttgtaaagca caatcaaaaa gatgatgtca tttgtttttc aaaactgaac tcgctttacg
361 agtagaattc tacgtgtaaa acacaatcaa gaaatgatgt catttgttat aaaaataaaa
421 gctgatgtca tgttttgcac atggctcata actaaactcg ctttacgggt agaattctac
481 gcgcgtcgat gtctttgtga tgcgcgcgac atttttgtag gttattgata aaatgaacgg
541 atacgttgcc cgacattatc attaaatcct tggcgtagaa tttgtcgggt ccattgtccg
601 tgtgcgctag catgcccgta acggacctcg tacttttggc ttcaaaggtt ttgcgcacag
661 acaaaatgtg ccacacttgc agctctgcat gtgtgcgcgt taccacaaat cccaacggcg
721 cagtgtactt gttgtatgca aataaatctc gataaaggcg cggcgcgcga atgcagctga
781 tcacgtacgt ccctcgtgtt ccgttcaagg acggtgttat cgacctcaga ttaatgttta
841 tcggccgact gttttcgtat ccgctcacca aacgcgtttt tgcattaaca ttgtatgtcg
901 gcggatgttc tatatctaat ttgaataaat aaacgataac cgcgttggtt ttagagggca
961 taataaaaga aatattgtta tcgtgttcgc cattagggca gtataaattg acgttcatgt
1021 tggatattgt ttcagttgca agttgacact ggcggcgaca agatcgtgaa caaccaagtg
1081 accatggcaa gctggagcca cccgcagttc gaaaagggtg cagatgacga cgacaaggta
1141 ccggatccga cgtcggtcga caagcttgcg gccgcaggag ctccgggctt ctcctcaatt
1201 tccgctcatc accaccatca tcaccatcac caccactaag tgattaacct caggttatac
1261 atatattttg aatttaatta attatacata tattttatat tatttttgtc ttttattatc
1321 gaggggccgt tgttggtgtg gggttttgca tagaaataac aatgggagtt ggcgacgttg
1381 ctgcgccaac accacctccc ttccctcctt tcatcatgta tctgtagata aaataaaata
1441 ttaaacctaa aaacaagacc gcgcctatca acaaaatgat aggcattaac ttgccgctga
1501 cgctgtcact aacgttggac gatttgccga ctaaaccttc atcgcccagt aaccaatcta
1561 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
1621 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
1681 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
1741 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
1801 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
1861 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
1921 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
1981 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
2041 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
2101 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
2161 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
2221 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
2281 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
2341 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
2401 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
2461 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
2521 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
2581 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
2641 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
2701 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
2761 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
2821 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
2881 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
2941 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
3001 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
3061 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
3121 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
3181 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
3241 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
3301 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
3361 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
3421 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
3481 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
3541 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
3601 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
3661 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
3721 tgattacgaa ttcccggg
//