LOCUS pTriEx-7 5124 bp DNA circular 1-SEP-2005
DEFINITION pTriEx-7 vector sequence.
SOURCE
ORGANISM
OMMENT VNTNAME|pTriEx-7|
COMMENT VNTREPLTYPE|Plasmid
COMMENT VNTEXTCHREPL|Bacteria
COMMENT VNTEXTCHREPL|Animal/Other Eukaryotic
FEATURES Location/Qualifiers
protein_bind 2186..2209
/vntifkey="31"
/label=Strep-Tag\II
terminator 2417..2623
/vntifkey="43"
/label=Rabbit\globin\terminator
rep_origin 3603..3603
/vntifkey="33"
/label=ori
misc_recomb 45..306
/vntifkey="86"
/label=Lef2
misc_recomb complement(343..948)
/vntifkey="86"
/label=603\ORF
repeat_unit 950..1008
/vntifkey="35"
/label=Artificial\Direct\Repeat-2
exon 1863..1930
/vntifkey="61"
/label=Exon\3\(Rabbit\Globin)
misc_recomb complement(2687..3392)
/vntifkey="86"
/label=1629
rep_origin 3435..4194
/vntifkey="33"
/label=pUC\ori
CDS complement(4205..5062)
/vntifkey="4"
/label=AmpR
promoter 1021..1597
/vntifkey="29"
/label=CMVie\enhancer/promoter
misc_feature 1129..1147
/vntifkey="21"
/label=Repeat\Element
misc_feature 1070..1087
/vntifkey="21"
/label=Repeat\Element
misc_feature 1264..1282
/vntifkey="21"
/label=Repeat\Element
misc_feature 1321..1338
/vntifkey="21"
/label=Repeat\Element
misc_feature 1426..1442
/vntifkey="21"
/label=Repeat\Element
misc_feature 1451..1469
/vntifkey="21"
/label=Repeat\Element
misc_feature 1489..1506
/vntifkey="21"
/label=Repeat\Element
misc_feature 1526..1543
/vntifkey="21"
/label=Repeat\Element
misc_feature 1579..1584
/vntifkey="21"
/label=Former\SacI
misc_feature 1610..1615
/vntifkey="21"
/label=Former\Hind\III
TATA_signal 1569..1575
/vntifkey="41"
/label=TATA\Box
exon 1598..1698
/vntifkey="61"
/label=Exon\1
misc_feature 1375..1380
/vntifkey="21"
/label=Former\Nco\I
terminator 2627..2674
/vntifkey="43"
/label=T7_TERM
promoter 1931..1947
/vntifkey="30"
/label=T7\Promoter
protein_bind 1952..1972
/vntifkey="31"
/label=lac\Operator
promoter 1986..2099
/vntifkey="29"
/label=p10\promoter\+\5'\UTR
misc_feature 2276..2290
/vntifkey="21"
/label=C-Term\Ek/LIC\overhang
protein_bind 2216..2230
/vntifkey="31"
/label=Ek\site
misc_binding 2300..2329
/vntifkey="20"
/label=10x\His-Tag
primer 2210..2293
/vntifkey="27"
/label=S-EkLIC\MCS
primer complement(2216..2299)
/vntifkey="27"
/label=AS-EkLIC\MCS
primer 2294..2342
/vntifkey="27"
/label=S-CTerm\10xHis
source 2114..2179
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57 BL/6"
/db_xref="taxon:10090"
/vntifkey="98"
sig_peptide 2114..2170
/vntifkey="94"
/label=Mouse\IgM\signal\seq
mat_peptide join(2171..2260,2465..2530)
/product="idiotypic anti-NP IgM heavy chain V-D-J"
/vntifkey="84"
/label=Mat_Peptide_1
misc_feature 2171..2179
/vntifkey="21"
/label=Misc_Feature_1
/note="variable region (aa 1-98)"
primer 2180..2202
/vntifkey="27"
/label=S-SigSeq\Strep\BstBI
primer 2113..2179
/vntifkey="27"
/label=S-mIgM\sigseq\NcoI
primer complement(2117..2184)
/vntifkey="27"
/label=AS-mIgM\sigseq\NcoI
BASE COUNT 1346 a 1147 c 1141 g 1490 t
ORIGIN
1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga
61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggcct
1021 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
1081 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
1141 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
1201 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagtc
1261 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
1321 ccttacggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgc
1381 tgatgcggtt ttggcagtac accaatgggc gtggatagcg gtttgactca cggggatttc
1441 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
1501 ttccaaaatg tcgtaataac cccgccccgt tgacgcaaat gggcggtagg cgtgtacggt
1561 gggaggtcta tataagcaga cgtcgtttag tgaaccgtca gatcactaga tgctttattg
1621 cggtagttta tcacagttaa attgctaacg ccagtctcga acttaacgtg cagaagttgg
1681 tcgtgaggca ctgggcaggt aagtatcggg ccctttgtgc ggggggagcg gctcggggct
1741 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
1801 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac
1861 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattggatc
1921 ggaccgaaat taatacgact cactataggg gaattgtgag cggataacaa ttccccggag
1981 ttaatccggg acctttaatt caacccaaca caatatatta tagttaaata agaattatta
2041 tcaaatcatt tgtatattaa ttaaaatact atactgtaaa ttacatttta tttacaatca
2101 aaggagatat accatgaaat tcagctgggt catgttcttc ctgatggcag tggttacagg
2161 ggtcaattca gaggttcagg caagctggag ccacccgcag ttcgaaaagg gtgcagatga
2221 cgacgacaag gtaccggatc cgacgtcggt cgacaagctt gcggccgcag gagctccggg
2281 cttctcctca atttccgctc atcaccacca tcatcaccat caccaccact aagtgattaa
2341 cctcaggtgc aggctgccta tcagaaggtg gtggctggtg tggccaatgc cctggctcac
2401 aaataccact gagatcgatc tttttccctc tgccaaaaat tatggggaca tcatgaagcc
2461 ccttgagcat ctgacttctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg
2521 gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt taaaacatca
2581 gaatgagtat ttggtttaga gtttggcaac atatgcccat atgtaactag cataacccct
2641 tggggcctct aaacgggtct tgaggggttt tttgctgaaa gcatgcggag gaaattctcc
2701 ttgaagtttc cctggtgttc aaagtaaagg agtttgcacc agacgcacct ctgttcactg
2761 gtccggcgta ttaaaacacg atacattgtt attagtacat ttattaagcg ctagattctg
2821 tgcgttgttg atttacagac aattgttgta cgtattttaa taattcatta aatttataat
2881 ctttagggtg gtatgttaga gcgaaaatca aatgattttc agcgtcttta tatctgaatt
2941 taaatattaa atcctcaata gatttgtaaa ataggtttcg attagtttca aacaagggtt
3001 gtttttccga accgatggct ggactatcta atggattttc gctcaacgcc acaaaacttg
3061 ccaaatcttg tagcagcaat ctagctttgt cgatattcgt ttgtgttttg ttttgtaata
3121 aaggttcgac gtcgttcaaa atattatgcg cttttgtatt tctttcatca ctgtcgttag
3181 tgtacaattg actcgacgta aacacgttaa atagagcttg gacatattta acatcgggcg
3241 tgttagcttt attaggccga ttatcgtcgt cgtcccaacc ctcgtcgtta gaagttgctt
3301 ccgaagacga ttttgccata gccacacgac gcctattaat tgtgtcggct aacacgtccg
3361 cgatcaaatt tgtagttgag ctttttggaa ttgcgatcgc ataacttcgt atagcataca
3421 ttatacgaag ttataagctc ggaacgctgc gctcggtcgt tcggctgcgg cgagcggtat
3481 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
3541 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
3601 ttttccatag gctccgcccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
3661 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
3721 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
3781 cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
3841 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
3901 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
3961 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
4021 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
4081 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
4141 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
4201 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
4261 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
4321 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
4381 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
4441 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
4501 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
4561 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
4621 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
4681 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
4741 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
4801 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
4861 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
4921 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
4981 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
5041 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
5101 gttattgtct catgtccgcg cgtt
//