Apoptosis Kits Selection Guide

Calbiochem and Oncogene Research Products offer a broad range of kits for the detection of Apoptosis. Find a kit that is suitable for your application using the dropdown lists below; or view our complete range of kits in the table beneath. If you are unable to find a kit that is suitable for your needs, please contact us with your requirements.

Click here to view other apoptosis-related products from Calbiochem and Oncogene Research Products

Select kit according to:

Sample Type	Method	Apoptotic Stage
- Select Sample Type -Biological FluidsCaspase EnzymeCells (Colorimetric Assay)Cells (Fluorescent Assay, green)Cells (Fluorescent Assay, Red)Cells (other types of assay)Cell LysatesCulture SupernatantFrozen Tissue SectionsParaffin Embedded Tissue SectionsPlasmaSerum	- Select Method -DNA ElectrophoresisELISA, plate reader (colorimetric assay)Enzyme Assay (colorimetric)Enzyme Assay (fluorescence)Flow CytometryFluorescence MicroscopyFluorescent Plate ReaderFluorimeterHistochemistryMagnetic SeparationMicroscopyWestern Blotting< /SELECT>	-Select Apoptotic Stage -General CaspaseGeneral Cell DeathGeneral, Early StageGeneral Early/Mid-StageSpecific mid/late-stageGeneral, Late StageSpecific, Late StageSpecific

Or view the complete range in the table below:

Kit Name	Cat. No.	Sample Type	Applicable Method(s)	General vs Specific	Feature Notes
Klenow FragEL™ DNA Fragmentation Detection Kit	QIA21	Cells, frozen tissue, sections, paraffin-embedded tissue sections	Microscopy	General, late-stage apoptosis	Colorimetric detection Detects DNA fragmentation through labeling ends of DNA breaks
TdT FragEL™ DNA Fragmentation Detection Kit	QIA33	Cells, frozen tissue, sections, paraffin-embedded tissue sections	Microscopy	General, late-stage apoptosis	Colorimetric detection TdT enzyme-based Detects DNA fragmentation through labeling ends of DNA breaks
Fluorescein FragEL™ DNA Fragmentation Detection Kit	QIA39	Cells, frozen tissue, sections, paraffin-embedded tissue sections	Fluorescence Microscopy Flow Cytometry (cells only)	General, late-stage apoptosis	Fluorometric detection Detects DNA fragmentation through labeling ends of DNA breaks
BrdUTP-FragEL™ DNA Fragmentation Detection Kit	QIA80	Cells, frozen tissue, sections, paraffin-embedded tissue sections	Microscopy	General, late- stage apoptosis	Colorimetric detection Detects DNA fragmentation through labeling ends of DNA breaks
Suicide Track™ DNA Ladder Isolation Kit	AM41	Cells	DNA Electrophoresis	General, late-stage apoptosis	Ethidium bromide stained agarose gel. Detects DNA fragmentation following DNA purification and gel electrophoresis
Nucleosome ELISA	QIA25	Cells	ELISA (plate reader)	General, late-stage apoptosis	Colorimetric detection. Alternative method for detecting DNA fragmentation. Detects DNA fragments bound to histone by sandwich ELISA
Annexin V FITC Apoptosis Detection Kit	PF032	Cells	Fluorescence Microscopy, Flow Cytometry	General, early-stage apoptosis	Green fluorescence. Includes propidium iodide to distinguish between early apoptotic and necrotic/late apoptotic cells. Detects annexin V binding to phosphatidylserine that becomes exposed on the membrane of apoptotic cells.
Annexin V-Biotin Apoptosis Detection Kit	PF036	Cells	Fluorescence Microscopy, Flow Cytometry	General, early-stage apoptosis	Uses biotinylated annexin V, leaving open the choice of fluorophore (researcher provides own streptavidin or avidin conjugate). Includes propidium iodide to distinguish between early apoptotic and necrotic/late apoptotic cells. Detects annexin V binding to phosphatidylserine that becomes exposed on the membrane of apoptotic cells.
Cu/Zn Superoxide Dismutase ELISA	QIA97	Culture supernatant Biological fluids	ELISA (plate reader)	Specific	Colorimetric detection. Detects SOD by sandwich ELISA.
Cell Death Detection (Nuclear Matrix Protein) ELISA Kit	QIA20	Culture supernatant	ELISA (plate reader)	General cell death	Colorimetric detection. Detects nuclear matrix protein released by dead/dying cells by sandwich ELISA.
Cytochrome c Release Apoptosis Assay Kit	QIA87	Cells	Western Blotting	General, early/mid-stage apoptosis	Leaves open option for detection (colorimetric vs chemiluminescence) Separation of mitochondrial and cytosolic fractions followed by cytochrome c detection by western blotting.
Cytochrome c ELISA Kit	QIA74	Cells Serum Plasma	ELISA (plate reader)	General, early/mid-stage apoptosis	Colorimetric detection. Detects cytochrome c from lysates or biological fluids by sandwich ELISA
Mitocapture™ Apoptosis Detection Kit	475866	Cells	Fluorescence Microscopy, Flow Cytometry	General early/mid-stage apoptosis	Fluorescent detection. Detects changes in the mitochondrial membrane using a dye that fluoresces differentially in healthy and apoptotic cells.
Apoptosis Cell Isolation Kit	QIA110	Cells	Magnetic Separation	General early/mid-stage apoptosis	Cell separation kit in which biotinylated annexin V is used to bind to the exposed phosphatidylserine at the surface of apoptotic cells. Streptavidin magnetic beads are used to separate these apoptotic cells from the whole cell population
Glutathione Apoptosis Detection Kit, Fluorometric	QIA89	Cells	Fluorimeter-based assay	General, early-stage apoptosis	Fluorescent detection. Detects changes in GST levels using a GST-specific dye.
Caspase Flow Cytometric Apoptosis Kit	QIA78	Cells	Enzyme Assay/Flow Cytometry	General Caspase	Fluorescent detection. Detects caspase activity using a general caspase substrate in cells.
Caspase Detection Kit (FITC-VAD-FMK)	QIA90	Cells	Fluorescence microscopy, flow cytometry, fluorescent plate reader	General Caspase	Fluorescent detection (green). Detects caspase activation using an irreversible, general, fluorescent caspase inhibitor to label caspases in the cell.
Caspase Activity Detection Kit (Red-VAD-FMK)	QIA92	Cells	Fluorescence microscopy, flow cytometry, fluorescent plate reader	General Caspase	Fluorescent detection (red). Detects caspase activation using an irreversible, general, fluorescent caspase inhibitor to label caspases in the cell.
Caspase-3 Activity Detection Kit (FITC-DEVD-FMK)	QIA91	Cells	Fluorescence microscopy, flow cytometry, fluorescent plate reader	Specific, mid/late-stage apoptosis	Fluorescent detection (green). Detects caspase-3 activation using an irreversible, fluorescent caspase-3 inhibitor to label caspase-3 in the cell
Caspase-3 Activity Detection Kit (Red-DEVD-FMK)	QIA93	Cells	Fluorescence microscopy, flow cytometry, fluorescent plate reader	Specific, mid/late-stage apoptosis	Fluorescent detection (red). Detects caspase-3 activation using an irreversible, fluorescent caspase-3 inhibitor to label caspase-3 in the cell.
Caspase-3 Intracellular Activity Assay Kit I (PhiPhiLux™G1D2)-Green	235430	Cells	Fluorescence microscopy, flow cytometry	Specific, mid/late-stage apoptosis	Fluorescent detection (green). Detects intracellular (intact cells) caspase-3 activity using specific caspase-3 substrate.
Caspase-3 Intracellular Activity Assay Kit II (PhiPhiLux™G2D2)-Red	235432	Cells	Fluorescence microscopy, flow cytometry	Specific, mid/late-stage apoptosis	Fluorescent detection (red). Detects intracellular (intact cells) caspase-3 activity using specific caspase-3 substrate.
Caspase-3 Activity Assay	QIA70	Cells	Enzyme assay (fluorescent plate reader)	Specific, late-stage apoptosis	Fluorescent Detection. Detects caspase-3 activity in cell lysates by cleavage of a caspase-3 specific substrate.
Caspase-3 Activity Assay for HTS	HTS02	Cells	Enzyme assay (fluorescent plate reader)	Specific, late-stage apoptosis	Fluorescent Detection. High throughput screening. Detects caspase-3 activity in cell lysates by cleavage of a caspase-3 specific substrate. Entire assay is carried out in the same well
Caspase-3 Cellular Activity Assay Kit	235419	Cells	Enyzme assay (plate reader)	Specific, late-stage apoptosis	Colorimetric detection. Detects caspase-3 activity in cell lysates by cleavage of a caspase-3-specific substrate.
Caspase-3 Assay Kit	235418	Caspase-3	Enzyme assay (plate reader)	Specific	Colorimetric detection. Detects caspase-3 activity by cleavage of a caspase-3 specific substrate. Useful for screening potential caspase-3 inhibitors.
Caspase-3 Immunoassay/Activity Kit, Fluorogenic	QIA107	Caspase-3	Enzyme assay (fluorescent plate reader)	Specific, late-stage apoptosis	Fluorogenic detection. Utilizes a caspase-3-specific antibody to capture active Caspase-3 from cell lystae prior to enzyme assay. Eliminates any potential background cleavage of substrate by other activated caspases in the sample.
Caspase-1 Assay Kit, Fluorometric	218791	Cells	Enzyme assay (fluorescent plate reader)	Specific, late-stage apoptosis	Fluorescent detection. Detects caspase-1 activity in cell lysates by cleavage of a caspase-1 specific substrate.
Caspase-1 Assay Kit, Colorimetric	218790	Cells	Enzyme assay (plate reader)	Specific, late-stage apoptosis	Colorimetric detection. Detects caspase-1 activity in cell lysates by cleavage of a caspase-1 specific substrate.
Caspase-1 Assay Kit	218734	Caspase-1	Enzyme assay (plate reader)	Specific	Colorimetric detection. Detects caspase-1 activity by cleavage of a caspase-1 specific substrate. Useful for screening potential caspase-1 inhibitors.
Caspase-2 Assay Kit, Fluorometric	218793	Cells	Enzyme assay (fluorescent plate reader)	Specific, late-stage apoptosis	Fluorescent detection. Detects caspase-2 activity in cell lysates by cleavage of a caspase-2 specific substrate.
Caspase-2 Assay Kit, Colorimetric	218792	Cells	Enzyme assay (plate reader)	Specific, late-stage apoptosis	Colorimetric detection. Detects caspase-2 activity in cell lysates by cleavage of a caspase-2 specific substrate.
Caspase-5 Assay Kit, Fluorometric	218801	Cells	Enzyme assay (fluorescent plate reader)	Specific, late-stage apoptosis	Fluorescent detection. Detects caspase-5 activity in cell lysates by cleavage of a caspase-5 specific substrate.
Caspase-5 Assay Kit, Colorimetric	218804	Cells	Enzyme assay (plate reader)	Specific, late-stage apoptosis	Colorimetric detection. Detects caspase-5 activity in cell lysates by cleavage of a caspase-5 specific substrate.
Caspase-6 Assay Kit, Fluorometric	218803	Cells	Enzyme assay (fluorescent plate reader)	Specific, late-stage apoptosis	Fluorescent detection. Detects caspase-6 activity in cell lysates by cleavage of a caspase-6 specific substrate.
Caspase-6 Assay Kit, Colorimetric	218802	Cells	Enzyme assay (plate reader)	Specific, late-stage apoptosis	Colorimetric detection. Detects caspase-6 activity in cell lysates by cleavage of a caspase-6 specific substrate.
Caspase-7 Immunoassay/Activity Kit, Fluorogenic	QIA108	Cell lysates	Enzyme assay (fluorescent plate reader)	Specific, late-stage apoptosis	Fluorogenic detection. Utilises a caspase-7-specific antibody to capture active caspase-7 from cell lysates prior to enzyme assay. Eliminates any potential background cleavage of substrate by other activated caspases in the sample.
Caspase-8 Activity Assay	QIA71	Cells	Enzyme assay (fluorescent plate reader)	Specific, mid/late-stage apoptosis	Fluorescent detection. Detects caspase-8 activity in cell lysates by cleavage of a caspase-8 specific substrate.
Caspase-8 Activity Assay for HTS	HTS03	Cells	Enzyme assay (fluorescent plate reader)	Specific, mid/late-stage apoptosis	Fluorescent detection. High throughput. Detects caspase-8 activity in cell lysates by cleavage of a caspase-8-specific substrate. Entire assay is carried out in the same well
Caspase-8 Assay Kit	218770	Caspase-8	Enzyme assay (plate reader)	Specific	Colorimetric detection. Detects caspase-8 activity by cleavage of a caspase-8 specific substrate. Useful for screening potential caspase-8 inhibitors.
Caspase-8 Detection Kit (FITC-IETD-FMK)	QIA113	Cells	Flow Cytometry, fluorescent plate reader, fluorescence microscopy	Specific mid/late stage apoptosis	Fluorescent detection (green). Detects caspase-8 activation using an irreversible fluorescent caspase-8 inhibitor to label caspase-8 in the cell.
Caspase-8 Detection Kit (Red-IETD-FMK)	QIA114	Cells	Flow Cytometry, fluorescent plate reader, fluorescence microscopy	Specific mid/late stage apoptosis	Fluorescent detection (red). Detects caspase-8 activation using an irreversible fluorescent caspase-8 inhibitor to label caspase-8 in the cell.
Caspase-9 Activity Assay	QIA72	Cells	Enzyme assay (fluorescent plate reader)	Specific, mid/late-stage apoptosis	Fluorescent detection. Detects caspase-9 activity in cell lysates by cleavage of a caspase-9 specific substrate
Caspase-9 Activity Assay for HTS	HTS04	Cells	Enzyme assay (fluorescent plate reader)	Specific, mid/late-stage apoptosis	Fluorescent detection. High throughput. Detects caspase-9 activity in cell lysates by cleavage of a caspase-9 specific substrate. Entire assay is carried out in the same well
Caspase-9 Assay Kit, Colorimetric	218824	Cells	Enzyme assay (plate reader)	Specific, mid/late-stage apoptosis	Colorimetric detection. Detects caspase-9 activity in cell lysates by cleavage of a caspase-9 specific substrate.
Caspase-9 Detection Kit (FITC-LEHD-FMK)	QIA115	Cells	Flow Cytometry, fluorescent plate reader, fluorescence microscopy	Specific mid/late stage apoptosis	Fluorescent detection (green). Detects caspase-9 activation using an irreversible fluorescent caspase-9 inhibitor to label caspase-9 in the cell.
Caspase-9 Detection Kit (Red-LEHD-FMK)	QIA116	Cells	Flow Cytometry, fluorescent plate reader, fluorescence microscopy	Specific mid/late stage apoptosis	Fluorescent detection (red). Detects caspase-9 activation using an irreversible fluorescent caspase-9 inhibitor to label caspase-9 in the cell.
Caspase-10 Assay Kit, Fluorometric	218811	Cells	Enzyme assay (fluorescent plate reader)	Specific, late-stage apoptosis	Fluorescent detection. Detects caspase-10 activity in cell lysates by cleavage of a caspase-10 specific substrate.
Caspase-10 Assay Kit, Colorimetric	218810	Cells	Enzyme assay (plate reader)	Specific, late-stage apoptosis	Colorimetric detection. Detects caspase-10 activity in cell lysates by cleavage of a caspase-10 specific substrate.
PARP Cleavage Detection Kit	512729	Cell lysates	Western Blot	Specific, late-stage apoptosis	Kit has been optimized for colorimetric detection. Specifically detects cleaved and uncleaved PARP using a specific antibody
PARP Cellular ELISA Kit	QIA105	Cells	ELISA (plate reader)	Excessive PARP activation can lead to necrosis	Colorimetric detection. Utilizes biotinylated NAD+ as a substrate for PARP resulting in the formation of biotinylated poly ADP ribose polymers following permeabilization of the cells. Indirect measure of PARP activity which is different from PARP cleavage that occurs in apoptosis.
Poly (ADP-ribose) Polymerase Histochemistry Kit	QIA106	Frozen tissue sections, Cells	Histochemistry (Microscopy)	Excessive PARP activation can lead to necrosis	Colorimetric detection. Utilizes biotinylated NAD+ as a substrate for PARP resulting in the formation of biotinylated poly ADP ribose polymers following permeabilization of the cells or tissue sections. Indirect measure of PARP activity which is different from PARP cleavage that occurs in apoptosis

 

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