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New ELISAs and PhosphoDetect ELISAs for Kinase Signaling
Faster and more sensitive detection compared to immunoblotting
 
Kinase ELISA and PhosphoDetect ELISA Procedure
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Quantitation of kinases or their substrates is an important part of disease and pathway analysis research. Analysis of kinases, phosphorylated kinases, and kinase substrate levels are important indicators of the signaling state of the cell. Pan or phospho-specific antibodies specifically and sensitively identify their targets in cell lysates or partially purified samples.

Immunoprecipitation using a pan antibody, followed by immunoblotting using a pan or phospho-specific detector antibody, provides sensitive detection of the target. However, this method is very time consuming and difficult to quantitate. Obtaining results from an immunoblot can take up to 2 days, with significant hands-on time.

Enzyme-linked immunosorbent assays (ELISAs) offer a faster and more sensitive method for quantitating kinases and their substrates in biological samples. ELISAs come with antibody-coated 96-well (strip) plates, a pan or phospho-specific detector antibody, a secondary antibody, and a protein standard. Quantitative detection of total or phosphorylated protein is obtained colorimetrically in about 4 hours.
 
ELISAs and PhosphoDetect ELISAs are:
•
Fast: Perform up to 96 assays in 4 hours
•
Sensitive: All reagents optimized for detection
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Complete: Contain all reagents needed, including standard for quantitation
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Specific: Target-specific; species specificity information provided
 

Detection of FAK (pTyr397) using the PhosphoDetect™ FAK (pTyr397) ELISA Kit is more sensitive than immunoblotting. The sensitivity of this ELISA was compared to immunoblotting using known quantities of FAK (pTyr397). The data show that the ELISA is more sensitive than immunoblotting. The bands shown in the immunoblot data were developed using anti-FAK (pTyr 397) and chemiluminescent detection.
 
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 Cat. No.
PhosphoDetect™ CREB (pSer133) ELISA Kit 96-well plate 96 Tests < 0.9 Units/ml 1.6-100 Units/ml 4 h human, mouse CBA072
CREB ELISA Kit 96-well plate 96 Tests < 0.025 ng/ml 0.156-10 ng/ml 4 h human, mouse CBA071
FAK ELISA Kit 96-well plate 96 Tests < 1.6 ng/ml 1.6-100 ng/ml 4 h human, mouse, rat CBA063
PhosphoDetect™ FAK (pTyr397) ELISA Kit 96-well plate 96 Tests < 0.9 Units/ml 1.6-100 Units/ml 4 h human, mouse CBA062
GSK-3b ELISA Kit 96-well plate 96 Tests < 0.26 ng/ml 0.39-25 ng/ml 4 h human, mouse, rat CBA068
PhosphoDetect™ GSK-3b (pSer9) ELISA Kit 96-well plate 96 Tests < 0.4 U/ml 1.6-100 U/ml 4 h human, mouse, rat CBA069
IkBa ELISA Kit 96-well plate 96 Tests < 30 pg/ml 62.5-4000 pg/ml 4 h human CBA064
PhosphoDetect™ IkBa (pSer32) ELISA Kit 96-well plate 96 Tests < 1.0 Units/ml 1.6-100 Units/ml 4 h human CBA065
PhosphoDetect™ c-Kit (pTyr823) ELISA Kit 96-well plate 96 Tests <1.0 Unit/ml 1.6 - 100 Units/ml 4 h human CBA075
c-Kit ELISA Kit 96-well plate 96 Tests < 15 pg/ml 19.5 - 1250 pg/ml 4 h human CBA074
MEK1 ELISA Kit 96-well plate 96 Tests < 0.1 ng/ml 0.16-10 ng/ml 4 h human, mouse, rat CBA070
PhosphoDetect™ c-Met (pTyr1230/1234/1235) ELISA Kit 96-well plate 96 Tests <0.25 Units/ml 1.56-100 Units/ml 4 h human CBA073
PhosphoDetect™ PRAS40 (pThr246) ELISA Kit 96-well plate 96 Tests < 0.9 units/ml 1.6-100 Units/ml 4 h human, mouse, rat CBA067
PRAS40 ELISA Kit 96-well plate 96 Tests <200 pg/ml 0.313-20 ng/ml 4 h human, mouse, rat CBA066
STAT1 ELISA Kit 96-well plate 96 Tests <0.27 ng/ml 0.3-20 ng/ml 4 h human CBA034
 
Related Products
Kinase Assays
PhosphoDetect™ Antibodies
 
 
 
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