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Novagen inNovations Newsletter

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inNovations 29, September 2008
1. Full issue, 7582 KB
2. iFOLD� Protein Refolding System 3
3. WideScreen™ Human Cancer Panels 1 and 2
4. NusA Fusion System
5. plEX/Bac™ -4 and plEx/Bac-5 Vectors
6. GOLD Standard Protein Expression Products
7. Product Introductions
8. How can we help you?
inNovations 28, July 2008
1. Full issue, 1939 KB
2. NanoJuice™ Transfection Kits
3. Gateway� Nova DEST™ Vectors
4. KOD Xtreme™ Hot Start DNA Polymerase
5. ProteoExtract� Transmembrane Protein Extraction Kit
6. Benzonase� Nuclease
7. GeneJuice� Transfection Reagent
inNovations 27, March 2008
1. Full issue, 1828 KB
2. WideScreen Assays
3. New Size Available - D-Tube™ Dialyzer Mega Kits
4. InnoZyme™ Insulysin/IDE Immunocapture Activity Assay
5. BugBuster� Protein Extraction Reagent
6. Answers to questions about Proteases for Fusion Tag Removal
7. His•Tag� Antibody HRP Conjugate Kit & Microbiology Media: Terrific Broth and 2xYT
inNovations 26, September 2007
1. Full issue, 988 KB
2. NEW Overnight Express™ Autoinduction NMR Media
3. Pellet Paint� Co-Precipitant for microarray work
4. Product Introductions
5. Introducing GST•Bind™ Fractogel� Cartridges
6. Unique new kits for phosphopeptide enrichment
7. Product Updates
8. Citation Spotlight
inNovations 25, February 2007
1. Full issue, 1104 KB
2. NEW ProteoExtract� Kits
3. Faster, Easier - proteins from insect cells
4. iFOLD™ Protein Refolding System 1
5. Rosetta™ (DE3) Competent Cells
6. Transfection reagent comparison
7. NEW! NoShift™ CREB, FOXO-1a Reagents
8. Answers to questions about Selecting E. coli cloning hosts
inNovations 24, August 2006
1. Full issue, 1104 KB
2. NEW ProteoExtract� Kits
3. Faster, Easier - proteins from insect cells
4. iFOLD™ Protein Refolding System 1
5. Rosetta™ (DE3) Competent Cells
6. Transfection reagent comparison
7. NEW! NoShift™ CREB, FOXO-1a Reagents
8. Answers to questions about Selecting E. coli cloning hosts
inNovations 23, May 2006
1. Full issue, 1685 KB
2. iFOLD™ Protein Refolding System 1
3. Microarray for human angiogenic cytokines
4. Introducing MAC cartridges
5. NovaTaq™ Master Mixes
6. NEW Transcription factor assays
7. Enrich samples for phosphoproteins
8. Origami™ B(DE3) Competent Cells
9. Answers to questions about ProteoPlex� Arrays
inNovations 22, November 2005
1. Full issue, 1899 KB
2. Strep•Tag� II fusion tag provides rapid one-step affinity purification
3. New ProteoEnrich™ chromatography kits
4. Protein refolding database
5. New NoShift™ Transcription Factor Assay Reagents
6. Answers to questions about the Strep•Tag�/Strep•Tactin� affinity purification products
inNovations 21, July 2005
1. Full issue, 1323 KB
2. (451 KB): Autoinduce protein expression without IPTG Overnight Express™ Instant TB Medium
3. (222 KB): Low sample volume murine cytokine microarray ProteoPlex™ Microarray
4. (109 KB): Comparison of Insect Cell Expression Systems: Baculovirus and InsectDirect™
5. (431 KB): New kits for dialysis and electroelution D-Tube™ Dialyzers and Electroelution Accessory Kit
6. (105 KB): Answers to questions about the Overnight Express Autoinduction Systems
inNovations 20, October 2004
1. Full issue, 1790 KB
inNovations 19, April 2004
1. Full issue, 2051 KB
inNovations 18, October 2003
1. Full issue, 1051 KB
2. (104 KB): Enhanced protein coexpression in E. coli
3. (178 KB): New coexpression vectors for expanded compatibilities in E. coli
4. (245 KB): A rapid method for simultaneously cloning two open reading frames for coexpression in E. coli
5. (203 KB): Identification of DNA binding proteins using the NoShift™ Transcription Factor Assay Kit
6. (135 KB): A novel buffer system for direct PCR from whole blood
7. (102 KB): Robotic solubility screening and purification of fusion proteins
8. (244 KB): New Products
inNovations 17, June 2003
1. Full issue, 1249 KB
2. (238 KB): Unattended high-density cell growth and induction of protein expression with the Overnight Express™ Autoinduction System
3. (206 KB): Fidelty and reliability in PCR using KOD Hot Start
4. (243 KB): Rapid microbial gene detection and amplification techniques using colony-direct PCR
5. (207 KB): Detection of Shiga toxin-producing E. coli using multiplex colony-direct PCR with KOD XL DNA Polymerase
6. (209 KB): Efficient production of recombinant plant chitinase with Novagen’s E. coli Origami™(DE3) Strain
7. (313 KB): Protein delivery into mammalian cells using ProteoJuice™ Protein Transfection Reagent
8. (404 KB): Improved immunohistochemical staining of fixed tissues
9. (244 KB): New Products
10. (470 KB): Product Spotlight: ProteoExtract™ Kits from Calbiochem
inNovations 16, March 2003
1. Full issue, 1013 KB
2. (249 KB): Greater yields of full-length proteins using the EcoPro™ T7 transcription/translation system
3. (321 KB): A novel high-level transient protein expression and purification system for Sf9 insect cells that facilitates automated screening
4. (273 KB): Automated solubility screening of recombinant proteins in a 96-well format
5. (359 KB): Simple,powerful promoter and enhancer analysis with the MightyLight™ System
6. (183 KB): MagPrep^� Bacterial Genomic DNA Kit: Efficient isolation of genomic DNA from Gram-positive and Gram-negative bacterial species
7. (312 KB): New Products
8. (53 KB): Application Spotlight: Extraction of proteins from three different yeast species using YeastBuster™ Protein Extraction Reagent
9. (141 KB): Application Spotlight: Use of BugBuster^� and Lysonase™ Reagents for efficient protein extraction from Gram-positive bacteria
inNovations 15, November 2002
1. Full issue, 932 KB
2. (357 KB): Coexpression of multiple target proteins in E. coli
3. (570 KB): Use of T7 phage display to clone RNA-binding cDNAs
4. (242 KB): Rapid and convenient extraction of nuclear proteins using the NucBuster™ Protein Extraction Kit
5. (245 KB):Application Feature: Protein Extraction Reagents Application Guide
6. (304 KB): YeastBuster™ Protein Extraction Reagent for fast,efficient extraction of proteins from yeast
7. (297 KB): Centrifugation-free protein extraction and purification from total cultures of baculovirus-infected insect cells
8. (435 KB): New Products
inNovations 14, July 2002
1. Full issue, 840 KB
2. (75 KB): Automated purification of recombinant proteins in a 96-well format with RoboPop Kits and robotic sample processing
3. (64 KB): Novel single-copy pETcoco™ vector with dual controls for amplification and expression
4. (51 KB): Targeted suppression of gene expression using siRNA and RiboJuice™ siRNA Transfection Reagent
5. (47 KB): pET-44 series for Nus•Tag™-enhanced protein solubility and improved purification with His•Mag™ Agarose Beads
6. (62 KB): Protocols to detect Cre recombinase expression in transgenic mouse tissues by immunostaining and Western blotting
7. (78 KB): Product Spotlight: Cleavage of fusion proteins
8. (147 KB): New Products
inNovations 13, November 2001
1. Full issue, 408 KB
2. (193 KB): Extraction and purification of proteins from E. coli without harvesting cells
3. (53 KB): Domain mapping of nuclear transport factor
4. (51 KB): Expression and purification of insect cadherin ectodomain using the pTriEx™-2 multisystem vector in Origami™ and Tuner™ cells
5. (62 KB): Use of glucose to control basal expression in the pET System
6. (74 KB): Preparation of protein samples for SDS-polyacrylamide gel electrophoresis: procedures and tips
inNovations 12, June 2001
1. Full issue, 565 KB
2. (125 KB): Overcoming the codon bias of E. coli for enhanced protein expression
3. (30 KB): Enhanced sensitivity of ChIP through the application of Pellet Paint^� Co-Precipitant
4. (36 KB): Simple,efficient extraction of protein from hepatocytes with CytoBuster™ Reagent
5. (59 KB): Sf9 insect cells and serum-free media adapted for different baculovirus applications
6. (71 KB): Western blotting and immunohistochemistry using the His•Tag^� Monoclonal Antibody
inNovations 11, June 2000
1. (140 KB): Mobius™ 1000 Plasmid Kits for convenient isolation of ultrapure plasmid DNA
2. (115 KB): Expression of soluble heterologous proteins via fusion with NusA protein
3. (102 KB): A superior E. coli coupled transcription-translation system for synthesis of full-length proteins from circular or linear DNA
4. (81 KB): New Products
inNovations 10, November 1999
1. (143 KB): pTriEx™-1 Multisystem Vector for protein expression in E. coli,mammalian,and insect cells
2. (102 KB): pETBlue™ Vectors: combining the utility of blue/white screening with the power of T7 promoter-based expression
3. (30 KB): FRET based homogenous assay of S•Tag™ fusion proteins
4. (43 KB): Efficient production of disulfide bonded proteins in the cytoplasm in ‘oxidizing’ mutants of E. coli
5. Nco I cloning site to express native target proteins >
6. (38 KB): Preparation of selenomethionine-labeled proteins using pET and E. coli
7. (59 KB): BugBuster™ and Benzonase^�: the clear solutions to simple,efficient extraction of E. coli proteins
8. (220 KB): Optimized automated sequencing with Pellet Paint^� NF Co-Precipitant
9. (133 KB): New Products
inNovations 9, January 1999
1. (135 KB): Non-chemical gene transfer into mammalian cells
2. (19 KB): Technical Tip: Use of "downstream" restriction enzyme cutters to generate any desired 4 bp 5′-overhang
3. (44 KB): Clonables™: a kit for fast,trouble-free ligation and transformation of any plasmid vector and insert
4. (13 KB): Technical Tip: Addition of glucose to growth media to reduce background expression in λDE3 lysogenic host strains
5. (59 KB): STP3™-Biotin: non-radioactive transcription/translation in a single tube
6. (52 KB): Pellet Paint^� Co-Precipitant minimizes false negatives in PCR assay
7. (66 KB): New Products
inNovations 8, September 1998
1. (160 KB): Simple,efficientin vitro protein expression with Single Tube Protein™ System 3
2. <(37 KB): Analysis of vitellogenin gene expression in individual pine weevils /a>
3. (42 KB): New pBAC transfer plasmids for production of secreted proteins
4. (59 KB): Ea4 insect cells for glycoprotein research
5. (79 KB): Efficient³²P-labeling of proteins expressed from pET-33b(+) for protein:protein interaction studies
6. (35 KB): Instant IMAC: pre-packed, pre-charged His•Bind Quick Cartridges and Columns
7. (78 KB): New Perfectly Blunt^� Kits and Quick Protocol: the fastest and most efficient PCR cloning system
8. (107 KB): New Products
inNovations 7, August 1997
1. (44 KB): Cellulose binding domains� A novel fusion technology for efficient,low cost purification and immobilization of recombinant proteins
2. (51 KB): New pET expression vectors generate fusion proteins with cellulose binding domains
3. (50 KB): New pBAC transfer plasmids for baculovirus expression of CBD fusion proteins
4. (89 KB): Purification and S•Tag detection of CBD fusion proteins
5. (69 KB): BacVector-3000: An engineered baculovirus designed for greater protein stability
6. (56 KB): New Products
inNovations 6, December 1996
1. (235 KB): T7Select Phage Display System: A Powerful New Protein Display System Based on Bacteriophage T7
2. (72 KB): Perfectly Blunt Cloning: A Superior Method for Cloning PCR Products or Any DNA
3. (27 KB): New Products
inNovations 5, August 1996
1. (114 KB): Immediate Early Baculovirus Vectors for Foreign Gene Expression in Transformed or Infected Insect Cells
2. (25 KB): Recombinant Enterokinase Cleavage of Fusion Proteins
3. (40 KB): Ligation Independent Cloning: Efficient Directional Cloning of PCR Products
4. (20 KB): Spectrophotometric Measurement of Nucleic Acids in the Presence of Pellet Paint™ Co-Precipitant
5. (26 KB): The Use of Pellet Paint^� as a Co-Precipitant in the Ribonuclease Protection Assay
6. (26 KB): Chemiluminescent Detection of S•Tag Fusion Proteins
7. (56 KB) New Products
inNovations 4a, May 1996
1. (152 KB): The BacVector System: Simplified Cloning and Protein Expression Using Novel Baculovirus Vectors
2. (48 KB): pET-33b(+): A pET Vector that Contains a Protein Kinase A Recognition Sequence
3. (46 KB): Lactose Fed-Batch Fermentation: A High-Yield Method Suitable for Use with the pET System
4. (62 KB): Pellet Paint^�: A Visible Nucleic Acid Carrier for Efficient,Error-Free Precipitation
5. (44 KB): New Products
inNovations 3, June 1995
1. (63 KB): A Novel Strategy for Directional Cloning of Random Primed cDNA
2. (24 KB): λSCREEN™: A High Efficiency cDNA Cloning Vector for Protein Expression,Purification and Subtractive Probe Analysis
3. (21 KB): Frequencies of Restriction Sites in Murine cDNA
4. (48 KB): pET TRX Fusion System for Increased Solubility of Proteins Expressed in E. coli
5. (18 KB): Frequently Asked Questions About cDNA Libraries
6. (414 KB): New Products
inNovations 2, November 1994
1. (127 KB): High-Yield Expression and Purification of Recombinant Peptides
2. (30 KB): Universal Western Blot Markers
3. (90 KB): Straight A�s™ mRNA Isolation System: Rapid,High-Quality Poly(A)+ RNA from Diverse Sources
4. (133 KB): New Products
inNovations 1, May 1994
1. (75 KB): The pET System: Your Choice for Expression
2. (136 KB): S•Tag™: A Multipurpose Fusion Peptide for Recombinant Proteins
3. (45 KB): pT7Blue T-Vector Stability
4. (59 KB): New Products