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A ROCK inhibitor permits survival of dissociated human embryonic stem cells
Kiichi Watanabe, Morio Ueno, Daisuke Kamiya, Ayaka Nishiyama, Michiru Matsumura,
Takafumi Wataya, Jun B Takahashi, Satomi Nishikawa, Shin-ichi Nishikawa, Keiko Muguruma & Yoshiki Sasai.
Nature Biotechnology. Volume 25 Number 6 June 2007. View PDF
 

 
Figure 1 The ROCK inhibitor Y-27632 markedly increases the cloning efficiency of hES cells (KhES-1) without affecting their pluripotency. (a,b) Low-density culture of dissociated hES cells in the absence (a) and presence (b) of 10 mM Y-27632 on MEF cells for 7 d. Almost all colonies were positive for ALP. Bars, 500 mm. (c) Ratios of ALP+ colonies to the number of initially seeded hES cells (**, P < 0.01 versus control, n = 3).
 
Cellular differentiation Regulators
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 Cat. No.
Cardiogenol C 217460
Cyclopamine, V. californicum 239803
Cyclopamine-KAAD 239804
Dispase, Bacillus polymyxa 322120
GSK-3 Inhibitor IX 361550
InSolution™ GSK-3 Inhibitor IX 361552
GSK-3 Inhibitor IX, Control, MeBIO 361556
GSK-3b Inhibitor XII, TWS119 361554
Jervine 420210
Purmorphamine 540220
trans-Retinoic Acid 554720
Reversine 554717
SANT-1 559303
g-Secretase Inhibitor I 565750
g-Secretase Inhibitor IX 565770
InSolution™ g-Secretase Inhibitor X 565771
Stem Cell Proliferation Inhibitor 569620
Vitamin D3, 1a, 25-Dihydroxy- 679101
Wnt Agonist 681665
Y-27632 688000
 
Product Citation
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Cat. No. 565770: Dando, J.S., et al. (2005) Notch/Delta4 Interaction in Human Embryonic Liver CD34+ CD38– Cells: Positive Influence on BFU-E Production and LTC-IC Potential Maintenance. Stem Cells. 23, 550-560.
 
 
 
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