Lyophilized. Lyophilized from 50 mM NaCl, 5 mM HEPES, pH 7.5. Recombinant, Bacillus anthracis anthrax lethal factor expressed in a specialized strain of B. anthracis. One of the three protein components of Anthrax toxin, lethal factor (LF) is a highly specific protease that cleaves members of the mitogen-activated protein kinase kinase (MAPKK) family. LF is comprised of four domains. Domain I binds the protective antigen to enter the target cell; domains II, III, and IV create a long groove to hold and cleave the MAPKK proteins. One unit is defined as the amount of enzyme required to catalyze the release of 1.0 pmole of cleaved MAPKKide™ substrate per min at 37°C, in 20 mM HEPES, pH 8.2. Purity: Single major band by SDS-PAGE.

Ref.: Brossier, F., and Mock, M. 2001. Toxicon 39, 1747. Friedlander, A.M. 2001. Nature 414, 160. Gupta, P., et al. 2001. Biochem. Biophys. Res. Commun. 284, 568. Pannifer, A.D., et al. 2001. Nature 414, 160. Pugsley, A.P. 1996. Proc. Natl. Acad. Sci. USA 93, 8155. Leppla, S.H. 1991. Methods Enzymol. 195, 153.