rhCKII

Liquid. In 350 mM NaCl, 20 mM Tris-HCl, 2 mM DTT, 1 mM EDTA, 0.1% TRITON^® X-100 Detergent, pH 7.5. AVOID FREEZE/THAW CYCLES. Recombinant, human casein kinase II expressed in E. coli. A cyclic nucleotide-independent serine/threonine protein kinase composed of catalytic a-subunits (M.W. 44 kDa) and regulatory b-subunits (M.W. 26 kDa) in an a₂b₂-tetramer. This material corresponds to one of several forms of native CKII. Suitable for in vitro phosphorylation of proteins and peptides, phosphopeptide synthesis, characterization of phosphorylating sites, screening of activators and inhibitors, and mechanistic studies. Activity: ≥500,000 units/ml. One unit is defined as the amount of enzyme that will catalyze the transfer of 1.0 pmol of phosphate to RRRADDSDDDDD per min at 30°C, pH 7.5, in a total volume of 25 µl. Note: 1 KU = 1000 units.

Ref.: Yamaguchi, Y., et al. 1998. Nucleic Acids Res. 26, 3854. Chester, N., and Marshak, D.R. 1993. Anal. Biochem. 209, 284. Russo, G.L., et al. 1992. J. Biol. Chem. 267, 20317.