ICErel-II, ICH-2, TX

Liquid. In 100 mM NaCl, 50 mM HEPES, 10 mM DTT, 1 mM EDTA, 10% glycerol, 0.5% CHAPS, pH 7.4. AVOID FREEZE/THAW CYCLES. Recombinant, human caspase-4 expressed in E. coli. Suitable for the study of enzyme regulation and kinetics, target substrate cleavage, and inhibitor screening. MW: 10000 and 19000. One unit is defined as the amount of enzyme that will cleave 1.0 pmol substrate Ac-LEHD-pNA (Cat. No. 218805) per min at 25°C, pH 7.4. Purity: ≥90% by SDS-PAGE. M.W. 10,000 and 19,000 (heterodimer).

Ref.: Kamada, S., et al. 1997. Oncogene, 15, 285. Nasir, J., et al. 1997. Mamm. Genome 8, 611. Nath, R., et al. 1996. Biochem. J. 319, 683.