ICErel-III, TY

Liquid. In 100 mM NaCl, 50 mM HEPES, 10 mM DTT, 1 mM EDTA, 10% glycerol, 0.5% CHAPS, pH 7.4. AVOID FREEZE/THAW CYCLES. Recombinant, human caspase-5 expressed in E. coli. Suitable for the study of enzyme regulation and kinetics, target substrate cleavage, and inhibitor screening. MW: 10000 and 22000. Specific activity: ≥100,000 units/mg protein. One unit is defined as the amount of enzyme that will cleave 1.0 pmol substrate Ac-LEHD-pNA (Cat. No. 218805) per min at 25°C, pH 7.4. Purity: ≥80% by SDS-PAGE. M.W. 10,000 and 22,000 (heterodimer).

Ref.: Schwartz, S. et al. 1999. Cancer Res. 59, 2995. Fucheu, C., et al. 1996. Eur. J. Biochem. 236, 207. Munday, N.A., et al. 1995. J. Biol. Chem. 270, 15870.