Mch2 Lyophilized. AVOID FREEZE/THAW CYCLES. Recombinant, human caspase-6 fused at the N- and C- termini to a His•Tag® sequence and expressed in E. coli as a single polypeptide. Subsequently undergoes spontaneous autoprocessing to yield the active enzyme. Useful for the study of enzyme regulation and kinetics, cleavage of target substrates, and inhibitor screening. MW: 11000 and 18000. Specific activity: ≥6000 units/mg protein. One unit is defined as the amount of enzyme that will cleave 1 nmol Ac-VEID-pNA (Cat. No. 218762) per h at 37°C, pH 7.2. Purity: ≥95% by SDS-PAGE. M.W. 11,000 and 18,000 (heterodimer). Ref.: LeBlanc, A., et al. 1999. J. Biol. Chem. 274, 23426. Stennicke, H.R., and Salvesen, G.S. 1997. J. Biol. Chem. 272, 25719. |
