Cyclin Dependent Kinase 5

Liquid. In 25 mM Tris-HCl, 100 µM DTT, 30% Glycerol, pH 7.4. AVOID FREEZE/THAW CYCLES. Recombinant, human Cdk5 fused to GST and expressed in E. coli.. Cdk5 together with p35 (or p25; Cat. No. 480741), or its isoform, p39 (or p30; Cat. No. 480742), forms an active enzyme complex. The active enzyme phosphorylates a variety of substrates (i.e. tau, neurofilament, retinoblastoma protein, synapsin, and etc) that are involved in various cellular functions, including differentiation, migration, and apoptosis. Aberrant Cdk5 activity has been implicated in a number of neurodegenerative diseases such as Alzheimer’s disease, amyotrophic lateral sclerosis (ALS), and Parkinson’s disease, where abnormal phosphorylation of tau and/or neurofilaments has been observed. One vial contains sufficient material for approximately 25 reactions. Specific activity: ≥100,000 units/mg protein. One unit is defined as the amount of enzyme, when reconstituted with either Nck5a (Cat. No. 480741) or Nck5ai (Cat. No. 480742), that will transfer 1.0 pmol phosphate to Histone H1 peptide (9-18) per min at 30°C, pH 7.2

Ref.: Lee, K.Y., et al. 1999. Neurosci. Res. 34, 21. Lee, K.Y., et al. 1997. J. Biol. Chem. 272, 5622. Lee, K.Y., et al. 1996. J. Biol. Chem. 271, 1538. Tang, D., et al. 1995. J. Biol. Chem. 270, 26897.