bA44G7
CDS1
CHEK2
CHK2 checkpoint homolog
RAD53

Liquid. In 270 mM sucrose, 150 mM NaCl, 50 mM Tris-HCl, 1 mM DTT, 0.1 mM EGTA, pH 7.5. AVOID FREEZE/THAW CYCLES. Recombinant, human checkpoint homologue kinase 2 (Chk2) fused to a His•Tag^® sequence at the N-terminus and expressed in S. frugiperda insect cells. Chk2 is involved in sensing DNA damage in eukaryotic cells. It plays an important role in the control of the cell cycle by blocking replication. Specific activity: ≥ 25,000 units/mg protein. One unit is defined as the amount of enzyme required to transfer 1 picomol phosphate to the synthetic peptide substrate KKKVSRSGLYRSPSMPENLNRPR per min at 30°C. Purity: ≥ 95% by SDS PAGE.

Ref.: Bartek, J., et al. 2001. Nat. Rev. Mol. Cell Biol. 12, 877.