Lyophilized. Recombinant, mouse caspase-3 fused at the N- and C- terminus to a His•Tag® sequence and expressed in E. coli as a single polypeptide. Subsequently undergoes spontaneous autoprocessing to yield the active enzyme. MW: 11000 and 17000. Specific activity: 300,000 units/mg protein. One unit is defined as the amount of enzyme that will cleave 1 nmol the substrate DEVD-pNA (Cat. No. 235400) per h at 37°C, pH 7.2. M.W. 11,000 and 17,000 (heterodimer) Ref.: van de Craen, M., et al. 1997. FEBS Lett. 403, 61. Juan, T.S., et al. 1996. Oncogene 13, 749. |
