Lyophilized. Recombinant, rat fused at the N- and C- terminus to a His•Tag® sequence and expressed in E. coli as a single polypeptide. Subsequently undergoes spontaneous yield the active enzyme. MW: 11000 and 17000. Specific activity: ≥9,000 units/mg protein. One unit is defined as the amount of enzyme that will cleave 1 nmol of the substrate DEVD-pNA (Cat. No. 235400) per h at 37°C, pH 7.2. Purity: ≥80% by SDS-PAGE. M.W. 11,000 and 17,000 (heterodimer) Ref.: Juan, T.S., et al. 1996. Oncogene 13, 749. |
