Liquid. In 20 mM sodium/potassium phosphate buffer, 100 µM EDTA, pH 7.25. AVOID FREEZE/THAW CYCLES. Recombinant, human cytochrome b5 expressed in E. coli. A membrane-bound hemoprotein that enhances the catalytic efficiency of some P450 isoforms, most notably members of the 3A subfamily. Serves as an electron buffer and is speculated to play either a direct role in electron transfer or an indirect role through allosteric interactions with P450. Suitable for in vitro drug metabolism and toxicology studies including reconstituted P450-dependent biotransformation reactions. Purity: ≥90% by SDS-PAGE. Heme content: ≥60 nmol/mg protein. CAS 9078-32-4. Ref.: Holmans, P.J., et al. 1994. Arch. Biochem. Biophys. 312, 554. Gillam, E.M.J., et al. 1993. Arch. Biochem. Biophys. 305, 123. |
