DAG Kinase

Lyophilized. Lyophilized from 300 mM NaCl, 250 mM imidazole, 50 mM sodium phosphate buffer, 1% n-decyl-b-D-maltopyranoside, pH 7.5. HYGROSCOPIC. Recombinant sn-1,2-diacylglycerol kinase fused to a His•Tag^® sequence and expressed in E. coli. Chromatographically purified preparation. Specific activity: ≥12 units/mg protein. One unit is defined as the amount of enzyme that will phosphorylate 1 µmol of DAG per min in the presence of cardiolipin at 25°C, pH 6.8. Purity: ≥90% by SDS-PAGE. EC 2.7.1.107.

Ref.: Sanders, C.R., et al. 1996. Biochemistry 35, 8610. de Chaffoy de Courcelles, D., et al. 1989. J. Biol. Chem. 264, 3274. van Veldhoven, P.P., et al. 1989. Anal. Biochem. 183, 177. de Chaffoy de Courcelles, D., et al. 1985. J. Biol. Chem. 260, 15762.