Endo F1
Endo-b-N-acetylglucosaminidase F1

Liquid. In 20 mM Tris-HCl, pH 7.5. DO NOT FREEZE. Recombinant, Elizabethkingia meningosepticum endoglycosidase F1 expressed in E. coli. Cleaves asparagine-linked or free oligomannose and hybrid, but not complex oligosaccharides. Core fucosylation reduces activity by 50 fold. Endo F1 will hydrolyze sulfate containing high mannose chains. It cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine. Less sensitive to protein conformation than N-Glycosidase F (Cat. No. 362185) and therefore is more suitable for deglycosylation of native proteins. Activity: ≥17 units/ml. Specific activity: ≥16 units/mg protein. One unit is defined as the amount of enzyme that will release N-linked oligosaccharides from 1.0 µmol denatured ribonuclease B per min at 37°C, pH 5.5. Contaminants: Proteases: none detected.. EC 3.2.1.96. Note: 1 mU = 1 milliunit.

Ref.: Tarentino, A.L., and Plummer, T.H. 1994. Methods Enzymol. 230, 44. Tarentino, A.L., et al. 1992. J. Biol. Chem. 267, 3868. Trimble, R.B., and Tarentino, A.L. 1991. J. Biol. Chem. 266, 1646.