Endo F2
Endo-b-N-acetylglucosaminidase F2

Liquid. In 25 mM NaCl, 10 mM sodium acetate buffer, pH 4.5. DO NOT FREEZE. Recombinant, Elizabethkingia meningosepticum endoglycosidase F2 expressed in E. coli. Cleaves asparagine-linked or free oligomannose and biantennary complex oligosaccharides from glycoproteins. It cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine. Less sensitive to protein conformation than N-Glycosidase F (Cat. No. 362185) and therefore is more suitable for deglycosylation of native proteins. This enzyme is not active above pH 6.0. Activity: ≥5 units/ml. Specific activity: ≥20 units/mg protein. One unit is defined as the amount of enzyme that will release N-linked oligosaccharides from 1 µmol porcine fibrinogen per min at 37°C, pH 4.5. Contaminants: N-acetylglucosaminidase, a- and b-galactosidase, a-mannosidase, neuraminidases, proteases: none detected. EC 3.2.1.96. Note: 1 mU = 1 milliunit.

Ref.: Reddy, A., et al. 1998. Glycobiology 8, 633. Tarentino, A.L., and Plummer, T.H. 1994. Methods Enzymol. 230, 44. Tarentino, A.L., et al. 1993. J. Biol. Chem. 268, 9702. Trimble, R.B., and Tarentino, A.L. 1991. J. Biol. Chem. 266, 1646.