Endo F3
Endo-b-N-acetylglucosaminidase F3

Liquid. In 20 mM Tris-HCl, pH 7.5. DO NOT FREEZE. Recombinant, Elizabethkingia meningosepticum endoglycosidase F3 expressed in E. coli. Cleaves asparagine-linked or free biantennary and triantennary complex, and Man₃GlcNAc oligosaccharides from glycoproteins. This enzyme cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide, generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine residue. Core fucosylation increases the activity of Endo F3 up to 40 fold. Exhibits no activity on high mannose and hybrid molecules. Less sensitive to protein conformation than N-Glycosidase F (Cat. No. 362185) and therefore is more suitable for deglycosylation of native proteins. Activity: ≥5 units/ml. Specific activity: ≥30 units/mg protein. One unit is defined as the amount of enzyme that will release N-linked oligosaccharides from 1.0 µmol porcine fibrinogen per min at 37°C, pH 5.5. Contaminants: Proteases: none detected. EC 3.2.1.96. Note: 1 mU = 1 milliunit.

Ref.: Tarentino, A.L., et al. 1995. Glycobiology 5, 599. Tarentino, A.L., and Plummer, T.H. 1994. Methods Enzymol. 230, 44. Tarentino, A.L., et al. 1993. J. Biol. Chem. 268, 9702. Trimble, R.B., and Tarentino, A.L. 1991. J. Biol. Chem. 266, 1646.