Death-associated protein kinase 1

Liquid. In 100 mM NaCl, 50 mM Tris-HCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol, pH 8.0. AVOID FREEZE/THAW CYCLES. Recombinant, human DAPK1 fused at the N-terminus to a GST-His₆-thrombin cleavage site sequence and expressed in S. frugiperda insect cells using a baculovirus expression system. DAPK1 is a Ca²⁺/calmodulin-dependent serine/threonine kinase that acts as a positive regulator of apoptosis. Specific activity: ≥50 U/mg protein. One unit is defined as the amount of enzyme required to transfer a nmol phosphate to IAKRRRLSSLRASTSKSESSQK peptide substrate per min at 30°C using variable concentrations of ATP (0.1-0.391 µM).

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