EPH-related tyrosine kinase receptor B4 Liquid. In 100 mM NaCl, 50 mM Tris-HCl, 5 mM DTT, 20% glycerol, pH 8.0 AVOID FREEZE/THAW CYCLES. Recombinant, human EPHB4 fused at the N-terminus to a GST-His6-thrombin cleavage site sequence and expressed in S. frugiperda insect cells using a baculovirus expression system. EPHB4 is a member of the tyrosine kinase family and is normally expressed in endothelial and neuronal cells. Ephrin-B ligands undergo phosphorylation on conserved inracellular tyrosines upon interaction with receptors. Aberrant expression of EPHB4 has been reported in prostate cancer and highly malignant breast cancers. Specific activity: ≥60 U/mg protein. One unit is defined as the amount of enzyme required to transfer 1 nmol phosphate to Poly(Glu,Tyr)4:1 per min at 30°C, using variable concentrations of ATP (100 nM to 1.71 µM). Ref.: Kumar, S.R., et al. 2006. Am. J. Pathol. 169, 279. Xia, G., et al. 2005. Cancer Res. 65, 4623. Sturz, A., et al. 2004. Biochem. Biophys. Res. Commun. 313, 80. Berclaz, G., et al. 2003. Ann. Oncol. 14, 220. Wang, H.U., et al. 1998. Cell 93, 741. |
