Focal Adhesion Kinase

Liquid. In 100 mM NaCl, 50 mM Tris-HCl, 15 mM reduced glutathione, 5 mM DTT, 20 % glycerol, pH 8.0. AVOID FREEZE/THAW CYCLES. Recombinant, human FAK fused at the N-terminus to a GST-thrombin cleavage site sequence and expressed in S. frugiperda insect cells using a baculovirus expression system. FAK is a non-receptor tyrosine kinase that plays a central role in cell spreading, differentiation, migration, cell death, and acceleration of the G1 to S phase transition of the cell cycle. Specific activity: ≥1.0 U/mg. One unit is defined as the amount of enzyme required to transfer 1 nmol phosphate to Poly(Ala,Glu,Lys,Tyr)_6:2:5:1 per min at 30°C using variable concentrations of ATP (0.1-0.987 µM).

Ref.: Fornaro, M., et al. 2000. Mol. Biol. Cell 11, 2235. Taylor, J, et al. 2000. J. Biol. Chem. 275, 19250. Vial, D., et al. 2000. J. Biol. Chem. 275, 28269. Ridyard, M.S., and Sanders, E.J. 1999. Anat. Embryol. 199, 1.