EIF2AK3 PKR

Liquid. In 50 mM Tris-HCl, 50 mM NaCl, 250 µM DTT, 100 µM EGTA, 100 µM EDTA, 100 µM PMSF, 25% glycerol, pH 7.5. AVOID FREEZE/THAW CYCLES. Recombinant, human PERK consisting of amino acids 563-1115 fused at the N-terminus to GST and expressed in E. coli. PERK phosphorylates the a-subunit of eukaryotic translation initiation factor 2 (EIF2a), which leads to its inactivation and reduces translation initiation. The active kinase is useful for the study of PERK regulation and for inhibitor screening. Specific activity: ≥26 nmol/min/mg protein. Kinase activity is measured as the molar amount of phosphate incorporated into SMAD3 at 30°C using a final concentration of 50 µM ATP. Purity: ≥90% by SDS-PAGE.

Ref.: Baltzis, D., et al. 2007. J Biol Chem. 43, 31675. Koumenis, C., et al. 2002. Mol Cell Biol. 21, 7405.