Insulin-like Growth Factor-1 Receptor Liquid. In 100 mM NaCl, 50 mM Tris-HCL, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol. AVOID FREEZE/THAW CYCLES. Recombinant, human IGF-1R fused at the N-terminus to a GST-His6-thrombin cleavage site sequence and expressed in S. frugiperda insect cells using a baculovirus expression system. IGF-1R belong to the tyrosine kinase receptor family that mediates cell survival and growth in response to its ligands, IGF-1 and IGF-2. It is also involved in the proliferation of transformed cells. Specific activity: ≥45 U/mg protein. One unit is defined as the amount of enzyme required to transfer 1 nmol phosphate to Poly(Glu,Tyr)4:1 per min at 30°C using variable concentrations of ATP (0.1-2.52 µM). Ref.: Abbot, A.M., et al. 1992. J. Biol. Chem. 267, 10759. |
