Hepatocyte growth factor receptor tyrosine kinase Liquid. In 100 mM NaCl, 50 mM Tris-HCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol, pH 8.0. AVOID FREEZE/THAW CYCLES. Recombinant, human c-MET (amino acids 956-1390) (Genebank entry NM_000245) fused at the N-terminus to a GST-His6-thrombin cleavage site sequence and expressed in S. frugiperda insect cells using a baculovirus expression system. c-Met is a receptor tyrosine kinase activated by hepatocyte growth factor (HGF)/scatter factor (SF). c-MET is a plasminogen-like protein thought to be a humoral mediator of liver regeneration. Specific activity: ≥30 U/mg protein. One unit is defined as the amount of enzyme required to transfer 1 nmol phosphate to Poly(Ala, Glu, Lys, Tyr)6:2:5:1 per min at 30°C, using variable concentrations of ATP (0.1-1.48 µM). Ref.: Palka, H.L., et al. 2003. J. Biol. Chem. 278, 5278. Vadnais, J., et al. 2002. J. Biol. Chem. 277, 48342. Crostella, L., et al. 2001. Oncogene 20, 3735. Guiton, M., et al. 1994. J. Biol. Chem 269, 30370. |
