Liquid. In 100 mM NaCl, 50 mM Tris-HCl, 15 mM reduced glutathione, 5 mM DTT, 20% glycerol, pH 8.0. AVOID FREEZE/THAW CYCLES. Recombinant, human cyclin dependent kinase 2 (Cdk2; amino acids 1-298) and recombinant, human cyclin A (CycA; amino acids 1-432), each fused to a GST-thrombin cleavage site sequence at their respective N-termini and co-expressed in S. frugiperda insect cells using a baculovirus expression system. MW: 60200 (Cdk2) and 74900 (cyclinA). Specific activity: ≥9 U/mg protein. One unit is defined as the amount of enzyme required to transfer 1 nmol phophate to Histone H1 per min at 30°C, using variable concetrations of ATP (100-683 nM). EC 2.7.1.112. Ref.: Ji, J.Y., et al. 2005. Development 132, 2501. |
