rhIL-3

Lyophilized. Carrier-free. Recombinant, human interleukin-3 expressed in E. coli. Stimulates the production and differentiation of macrophages, neutrophils, basophils, mast cells, and eosinophils. As a hematopoietic colony-stimulating factor, IL-3 interacts with very early multipotent hematopoietic progenitor cells. IL-3 supports colony formation in soft agar of bone marrow cells of the granulocyte, macrophage, megakaryocyte, and erythrocyte lineages. IL-3 is reported to suppress the secretion of IL-12 and shift dendritic cells from the Th1 to Th2 phenotype. Biological activity: ED₅₀ = 100-250 pg/ml as measured in a cell proliferation assay with a human, IL-3 -dependent cell line.. Purity: ≥95% by SDS-PAGE. Contaminants: Endotoxin: ≤100 pg/mg IL-3.

Merck Index: 14, 4995
Ref.: Ebner, S., et al. 2002. J. Immunol. 168, 6199. Theodossiou, C., et al. 1994. Cancer 74, 2808. Dercksen, M.W., et al. 1993. Br. J. Cancer 68, 996. Miyajima, A., et al. 1993. Blood 82, 1960. Kitamura, T., et al. 1989. J. Cell Physiol. 140, 323. Schrader, J.W. 1986. Annu. Rev. Immunol. 4, 205.