Insulin Receptor b Liquid. In 50 mM Tris-HCl, 1 mM DTT, pH 7.5. AVOID FREEZE/THAW CYCLES. Recombinant, human IR b-subunit (amino acids 941-1343) fused to GST and expressed in S. frugiperda insect cells using a baculovirus expression system. Specific activity: ≥50 units/mg protein. One unit is defined as the amount of enzyme required to transfer 1 nmol phosphate to poly[Glu:Tyr] 4:1 substrate per min at 25°C. Purity: ≥80% by SDS-PAGE. Ref.: Al-Hasani, H., et al. 1994. FEBS Lett. 349, 17. |
