Liquid. Sterile-filtered in 100 mM NaCl, 20 mM Tris, 1 mM cysteine, 50 µg BSA/µg IL-10, pH 8.0. AVOID FREEZE/THAW CYCLES. Recombinant, mouse interleukin-10 expressed in E. coli. Pleiotropic cytokine that can exert either immunosuppressive or immunostimulatory effects on a variety of cell types. Potent inhibitor of monocyte/macrophage proliferation. Known to reduce neuronal degeneration following CNS injury by blocking glutamate-induced caspase-3 activation. Biological activity: ED50 = 0.3-0.6 ng/ml as measured in a cell proliferation assay using a mouse mast cell line, MC/9-2. Purity: ≥97% by SDS-PAGE. Contaminants: Endotoxin: >100 pg/µl IL-10. Ref.: Bachis, A., et al. 2001. J. Neurosci. 21, 3104. Fleming, S.D., et al. 1996. J. Immuol. 156, 1143. Wang, C.Q., et al. 1996, J. Cell Physiol. 166, 305. Niiro, H., et al. 1994. Int. Immunol. 6, 661. Moore, K., et al. 1993. Annu. Rev. Immunol. 11, 165. Vieira, P., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 1172. Moore, K., et al. 1990. Science 248, 1230. |
