SAPK1a Liquid. In 100 mM NaCl, 50 mM Tris-HCl, 1 mM DTT, 50% glycerol, pH 8.5. AVOID FREEZE/THAW CYCLES. Recombinant, human JNK2a2 fused to a His•Tag® sequence and expressed in E. coli. It is capable of autophosphorylation and suitable for labeling JNK2a2 substrates. Specific activity: ≥100 units/mg protein. One unit is defined as the amount of enzyme that will catalyze the transfer of 1.0 pmol phosphate to a c-Jun protein substrate per min at 30°C. Purity: ≥95% by SDS-PAGE. Contaminants: Proteases: none detected. Ref.: Kallunki, T., et al. 1994. Genes Dev. 8, 2996. Su, B., et al. 1994. Cell 77, 727. |
