Importin-b1 Liquid. In 100 mM potassium acetate, 20 mM HEPES-KOH, 2 mM DTT, 1 mM EGTA, 1 mM magnesium acetate, pH 7.3. AVOID FREEZE/THAW CYCLES. Recombinant, human karyopherin-b expressed in E. coli. A nuclear transport protein that binds karyopherin-a2. Karyopherin-b1 along with karyopherin-a2, Ran, and an energy source, supports nuclear import of proteins containing nuclear localization signals (NLS). Suitable for use in nuclear import and binding studies. Biological activity: maximal import of a rhodamine labeled BSA-NLS substrate in digitonin-permeabilized HeLa cells with ≤0.3 µM karyopherin-b1 added to the import mix. Purity: ≥60% by SDS/PAGE. Ref.: Takizawa, C.G., et al. 1999. Proc. Natl. Acad. Sci. USA 96, 7938. Schwoebel, E.D., et al. 1998. J. Biol. Chem. 273, 35170. Chi, N.C., et al. 1995. J. Cell Biol. 130, 265. Görlich, D., et al. 1995. Curr. Biol. 5, 383. |
