Liquid. In 100 mM NaCl, 50 mM Tris-HCl, 2 mM DTT, 100 µM EDTA, 50% glycerol, 0.05% NP-40, pH 7.5. AVOID FREEZE/THAW CYCLES. Recombinant, human c-Kit (amino acid 544-976) fused to a His•Tag® squence and expressed in S. frugiperda insect cells. Ligand binding to c-Kit, a tyrosine kinase, results in diverse cellular responses such as differentiation, proliferation, growth, survival, adhesion, and chemotaxis. Specific activity: ≥12 units/mg protein. One unit is defined as the amount of enzyme that will transfer 1.0 nmol phosphate to poly(Glu,Tyr) 4:1 substrate per min at 30°C, pH 7.5 Purity: ≥90% by SDS-PAGE. EC 2.7.11. Ref.: Voytyuk, O., et al. 2003. J. Biol. Chem., 278, 9159. Wehrle-Haller, B., and Imhof, B.A. 2001. J. Biol. Chem. 276, 12667. |
