Liquid. In 100 mM NaCl, 50 mM Tris-HCl, 1 mM DTT, 50 µM EDTA, 50% glycerol, 0.05% NP-40, pH 7.5. AVOID FREEZE/THAW CYCLES. Recombinant, human Lck fused to a His•Tag® sequence and expressed in S. frugiperda insect cells. Lck is a member of the Src tyrosine kinases that plays a role in T cell activation and in the regulation of mitochondrial apoptosis pathways. Specific activity: ≥100 units/mg protein. One unit is defined as the amount of enzyme that will transfer 1.0 nmol phosphate to poly(Glu,Tyr) 4:1 substrate per min at 30°C, pH 7.5 Purity: ≥90% by SDS-PAGE. EC 2.7.11. Ref.: Belka, C., et al. 2003. Oncogene 22, 176. Holdorf, A.D., et al. 2002. Nature Immunol. 3, 259. Rao, N., et al. 2002. Proc. Natl. Acad. Sci. USA 99, 3794. Marth, J.D., et al. 1988. Nature 332, 171. |
