Mouse Double Minute 2, Human, Recombinant, E. coli

Liquid. In 100 mM KCl, 20 mM Tris-Cl, 1 mM DTT, 0.2 mM EDTA, 20% glycerol, pH 8.0. AVOID FREEZE/THAW CYCLES. Recombinant, human MDM2 expressed in E. coli and purified by affinity chromatography using FPLC. Useful for in vitro transcription assays and DNA-protein and protein interaction assays. MDM2 is an E3 ubiquitin ligase that was first identified in mouse as a small acentromeric extrachromosomal element. It was then shown to become oncogenic upon amplification and tumorigenic when over-expressed in NIH3T3 and Rat2 cells. MDM2 overexpression is known to promote tumor development by inhibiting wild-type p53 transcriptional activation. Biological activity: 20-200 ng is sufficient for an in vitro protease assay and 100 ng is sufficient for a protein-protein interaction assay.. One unit is defined as 1 ng purified protein. Purity: > 95% by SDS-PAGE.

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