Hepatitis C Virus NS3/4A Protease, Recombinant, E. coli. Liquid. In 500 mM KCl, 20 mM Tris-HCl, 1 mM DTT, 0.2 mM EDTA, 20% glycerol, pH 8.0. AVOID FREEZE/THAW CYCLES. Recombinant, human HCV NS3/4A protease expressed in E. coli and purified by affinity chromatography using FPLC. HCV is a positive, single-stranded RNA virus of the Flaviviridae family that encodes a polyprotein precursor of about 3,000 amino acids. It is a common cause of chronic liver disease. HCV NS3/4A is an essential enzyme for viral replication. It is believed that HCV NS3/4A protease could play an important role in a treatment of hepatitis C infection. Biological activity: 20-200 ng is sufficient for an in vitro protease assay. One unit is defined as 1 ng of purified protein. Purity: ≥95% by SDS-PAGE. Ref.: De Francesco, R., et al. 2003. Antiviral Res. 58, 1. Lamarre, D., et al. 2003. Nature 426, 186. Kolykhalov, A. et al. 2000. J. Virol. 74, 2046. Blight, K. J., et al. 1998. Antiviral Ther. 3, 71. Alter, M. J. 1997. Hepatology 26, 62S. Bartenschlager, et al. 1995. J. Virol. 69, 7519. Lin, C., and Rice, C. M. 1995. Proc. Natl. Acad. Sci. U.S.A. 92, 7622. Grakoui, A., et al. 1993. J. Virol. 67, 2832. Hijikata, M., et al. 1993. Proc. Natl. Acad. Sci. U.S.A. 90, 10773. Tomei, L., et al. 1993. J. Virol. 67, 4017. |
