2′/3′-O-(N-Methylanthraniloyl)-guanosine-5′-(g-thio)triphosphate, Triethylammonium Salt
2′/3′-O-(N-Methylanthraniloyl)-g-thioguanosine-5′-triphosphate, Triethylammonium Salt
mGTPgS Liquid. A 5 mM solution in H2O. PROTECT FROM LIGHT. A more lipophilic, fluorescent derivative of the GTP-hydrolysis-resistant GTP analog, GTPgS (Cat. No. 371545). Useful for investigating the interactions of low molecular weight GTP-binding proteins with their specific effector proteins. Acts as a potent, competitive inhibitor of adenylyl cyclase (AC; Ki = 53 nM in forskolin/Mn2+-stimulated AC in S49 cyc- membranes). Shown to bind to Go-proteins with high affinity, Gsa-Gia-proteins with low affinity, and ineffective at activating Ga. Purity: ≥95% by HPLC. Ref.: Gille, A., and Seifert, R. 2003. J. Biol. Chem. 278, 12672. Remmers, A.E., et al. 1999. Biochemistry 38, 13795. Lan, K.L., et al. 1998. Biochemistry 37, 837. Remmers, A.E. 1998. Anal. Biochem. 257, 89. Remmers, A.E., and Neubig, R.R. 1996. J. Biol. Chem. 271, 4791. Remmers, A.E., et al. 1994. J. Biol. Chem. 269, 13771. |
