Collagenase-3

Liquid. In 150 mM NaCl, 50 mM Tris-HCl, 5 mM CaCl₂, pH 7.5. AVOID FREEZE/THAW CYCLES. Full-length recombinant, human MMP-13 fused to a His•Tag^® sequence and expressed in S. frugiperda insect cells. This 452 amino acid proenzyme contains the N-terminal propeptide, the Ca²⁺- and Zn²⁺-binding catalytic domain, the hinge region, and the C-terminal hemopexin domain. Hydrolyzes collagen type II 5 to 6 times faster than collagens Type I and III. Exhibits high activity towards gelatin and degrades a₁-antichymotrypsin and plasminogen activator inhibitor-2 (PAI-2). Specific activity: ≥50 mU/mg protein. One unit is defined as the amount of APMA-activated enzyme that will hydrolyze 1.0 µmol MCA-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH₂ (Cat. No. 03-32-5032) per min at 37°C, pH 7.5. Purity: ≥90% by SDS-PAGE. Note: 1 mU = 1 milliunit.

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