ERK1
MAPK3
p44MAPK Liquid. In 100 mM NaCl, 50 mM Tris-HCl, 1 mM DTT, 50% glycerol, pH 8.5. AVOID FREEZE/THAW CYCLES. Recombinant, human MAP Kinase 1 fused to a His•Tag® sequence and expressed in E. coli. Highly active form produced by phosphorylation of the purified MAP Kinase in vitro with MEK1. Suitable for labeling MAP Kinase substrates. Specific activity: ≥6000 units/mg protein. One unit is defined as the amount of enzyme that will catalyze the transfer of 1.0 pmol phosphate to myelin basic protein (MBP) per min at 30°C. Purity: ≥95% by SDS-PAGE. Contaminants: Proteases: none detected. Ref.: Charest, D.L., et al. 1993. Mol. Cell Biol. 13, 4679. |
