ERK2 MAPK2 p42MAPK Liquid. In 100 mM NaCl, 50 mM HEPES, 1 mM DTT, 100 µM Na2-EDTA, 50% glycerol, 0.01% BRIJ® 35 detergent, pH 7.5. AVOID FREEZE/THAW CYCLES. Recombinant, mouse MAP Kinase 2 expressed in E. coli. A serine/threonine protein kinase that acts at the end of a protein kinase cascade linking signals from cell surface receptor tyrosine kinases to cytoplasmic and nuclear events. This protein was co-expressed with MEK and is phosphorylated and active. Activity: ≥5000 units/ml. One unit is defined as the amount of enzyme that will catalyze the transfer of 1.0 pmol phosphate to myelin basic protein per min at 30°, pH 7.5. Purity: ≥90% by SDS-PAGE. Contaminants: MEK, phosphatase, protease: none detected.. Ref.: Fukunaga, K., and Miyamoto, E. 1998. Mol. Neurobiol. 16, 79. Wu, J., et al. 1993. Mol. Cell. Biol. 13, 4539. |