ERK2
MAPK2
p42MAPK Liquid. In 150 mM NaCl, 50 mM Tris, 1 mM DTT, 50% glycerol, pH 8.5. AVOID FREEZE/THAW CYCLES. Recombinant, human MAP Kinase 2 fused to a His•Tag® sequence and expressed in E. coli. Highly active form produced by phosphorylation of the purified MAP Kinase in vitro with MEK1. Suitable for labeling MAP Kinase substrates. Specific activity: ≥5500 units/mg protein. One unit is defined as the amount of enzyme that will catalyze the transfer of 1.0 pmol phosphate to myelin basic protein (MBP) per min at 30°C. Purity: ≥95% by SDS-PAGE. Contaminants: Proteases: none detected. Ref.: Lew, J. 2003. Biochemistry 42, 849. Johnson, G.L., and Lapadat, R. 2002. Science 298, 1911. Koistinaho, M., and Koistinaho, J. 2002. Glia 40, 175. |
