MEK6
Mitogen Activated Protein Kinase kinase 6
SAPKK3 In 270 mM sucrose, 150 mM NaCl, 50 mM Tris-HCl, 1 mM DTT, 0.1 mM EGTA, pH 7.5. AVOID FREEZE/THAW CYCLES. Recombinant, human MKK6 fused to GST at the N-terminus to GST and expressed in E. coli. MKK6 is a member of the dual specificity protein kinase family, which functions as a mitogen-activated protein (MAP) kinase kinase. It is suitable for labeling MAP kinase substrates. Specific activity: ≥ 50,000 units/mg protein. One unit is defined as the amount of enzyme that will activate p38alpha/SAPK2a (0.5 mg/ml) by 1 unit/min using 125 µM ATP at 30°C. One unit of p38a/SAPK2a activity is defined as the amount of enzyme that will transfer 1 pmol phosphate to myelin basic protein per min at 30°C. Purity: ≥ 90% by SDS PAGE. Ref.: Chabaud-Riou, M. and Firestein, G.S. et al. 2004. Am. J. Pathol. 164, 177. Zhu, X., et al. 2001. J. Neurochem. 79, 311. |
