ERK Kinase 1
MAP Kinase Kinase
Microtubule-associated protein 2 kinase Liquid. In 270 mM sucrose, 150 mM NaCl, 50 mM Tris-HCl, 1 mM DTT, 0.1 mM EGTA, pH 7.5 AVOID FREEZE/THAW CYCLES. Recombinant, human MEK1 fused to GST at the N-terminus and expressed in E. coli. MEK1 is a member of a dual-specificity protein kinase family that phosphorylates threonine and tyrosine residues on ERK 1 and 2. Specific activity: ≥ 250,000 units/mg protein. One unit is defined as the amount of enzyme that activates ERK1 (0.3 mg/ml) by 1 unit/min using 100 µM ATP at 30°C. One unit ERK1 activity is defined as that amount of enzyme required to transfer 1 pmol phosphate to myelin basic protein, in the presence of 125 µM ATP, per min at 30°C. Purity: ≥80% by SDS PAGE. Ref.: Allen, L.F., et al. 2003. Semin. Oncol. 30, 105. Schramek, H., et al. 1997. J. Biol. Chem. 272, 11426. Alessandrini, A., et al. 1996. J. Biol. Chem. 271, 31612. |
