MMP-14 Catalytic Domain

Liquid. In 150 mM NaCl, 50 mM Tris-HCl, 5 mM CaCl₂, pH 7.5. AVOID FREEZE/THAW CYCLES. Recombinant, human pro-MT1-MMP expressed in E. coli and activated to yield the active catalytic domain (amino acids 89-265). Degrades fibrillar collagen Types I, II, and III, as well as other extracellular matrix proteins, and activates MMP-2 and MMP-13 resulting in enhanced proteolysis of the matrix for cell migration and proliferation. Specific activity: ≥140 mU/mg protein. One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol MCA-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH₂ (Cat. No. 03-32-5032) per min at 37°C, pH 7.5. Purity: ≥90% by SDS-PAGE. Note: 1 mU = 1 milliunit.

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