MMP-16 Catalytic Domain Liquid. In 150 mM NaCl, 50 mM Tris-HCl, 5 mM CaCl2, 1% glycerol, 0.1% TRITON® X-100 Detergent, pH 7.5. AVOID FREEZE/THAW CYCLES. Recombinant, human pro-MT3-MMP expressed in E. coli as a periplasmic protein and activated to yield the active catalyic domain (amino acids 91-267). MT3-MMP is used to study the activation of MMP-2 and the degradation of extracellular matrix proteins. Specific activity: ≥75 mU/mg protein. One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol MCA-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 (Cat. No. 03-32-5032) per min at 37°C, pH 7.5. Purity: ≥80% by SDS-PAGE. Note: 1 mU = 1 milliunit. Ref.: Zhao, H., et al. 2003. J. Biol. Chem. 279, 8592. Matsumoto, S., et al. 1997. Biochim. Biophys. Acta 1354, 159. Puente, X.S., et al. 1996. Cancer Res. 56, 944. Takino, T., et al. 1995. J. Biol. Chem. 270, 23013. Knight, C.G., et al. 1992. FEBS Lett. 296, 263. |