Sialidase L Liquid. In 20 mM phosphate buffer, 2% glycerol, pH 7.0. AVOID FREEZE/THAW CYCLES. Recombinant, Macrobdella decora a2,3-Neuraminidase expressed in E. coli. Catalyzes the hydrolysis of a2,3-linked N-acetylneuraminic acid from complex oligosaccharides. Will catalyze the cleavage of a2,3-linkages from a1-acid glycoprotein, fetuin, keratan sulfate, sialyl Lewis x, and 3′-sialyllactose, yielding 2,7-anhydro-Neu5Ac instead of “free Neu5Ac” as the hydrolysis product. Specific activity: ≥2000 U/mg protein. One unit is defined as the amount of enzyme that will catalyze the release of 1 nmol methylumbelliferone from 2′-(4-Methylumbelliferyl)-D-N-acetylneuraminic Acid (Cat. No. 474495) per min at 37°C, pH 5.5. Contaminants: Ceramide glycanase, a- and b-galactosidase, b-hexosaminidase, a- and b-mannosidase, proteases: none detected. EC 3.2.1.18, RTECS QQ3450000, CAS 9001-67-6. Note: 1 mU = 1 milliunit. Ref.: Chou, M.-Y., et al. 1996. J. Biol. Chem. 271, 19219.
R: 20-36/37/38-42/43; S: 26-36 |
