rmOPN Lyophilized. Lyophilized from 0.2 µm sterile-filtered PBS, 50 µg BSA/µg rmOPN. Recombinant, mouse osteopontin fused to a His•Tag® sequence and expressed in NSO cells. A secreted, highly acidic, Ca2+-binding, RGD-containing, phosphorylated glycoprotein. Chemotactic for macrophages, smooth muscle cells, endothelial cells, and glial cells. Has been shown to inhibit nitric oxide production and cytotoxicity by activated macrophages. Plays a significant role in inducing granulomas of various origin. OPN is also involved in the migration of embryonic fibroblasts, macrophages, and metastatic cells. Biological activity: ~200 ng immobilized osteopontin/well will mediate the adhesion of ≥25% of the 293 cells plated at 105 cells/well. Purity: ≥95% by SDS-PAGE. Contaminants: Endotoxins: ≤100 pg/µg rmOPN. M.W. 65,000 and 30,000 under reducing conditions. Ref.: Xie, Y., et al. 2001. Kidney Int. 60, 1645. Chiba, S., et al. 2000. Microbiol. Immunol. 44, 319. Miyazaki, Y., et al. 1990. J. Biol. Chem. 265, 14432. |
