Liquid. In 100 mM NaCl, 50 mM HEPES, 1 mM DTT, 100 µM EDTA, 50% glycerol, 0.01% BRIJ® 35 Detergent, pH 7.5. AVOID FREEZE/THAW CYCLES. Recombinant, human p34Cdk1/cyclin B expressed in S. frugiperda insect cells. This kinase is a major regulator of the eukaryotic cell cycle. As a monomer, Cdk1 is inactive and requires association with cyclin B and phosphorylation of Thr161 to form an active complex. MW: 34000(p34Cdk1) and 55000(cyclinB). Activity: ≥20,000 U/ml. One unit is defined as the amount of enzyme that catalyzes the transfer of 1 pmol of phosphate to histone H1 (10 µM) in 1 min at 30°C, pH 7.5. Contaminants: Protease, phosphatase: none detected. Ref.: Kobayashi, H., et al. 1994. J. Biol. Chem. 269, 29153. Atherton-Fessler, S., et al. 1993. Mol. Cell. Biol. 13, 1675. |
