rPrPC Liquid. In 10 mM sodium acetate buffer, pH 4.0. AVOID FREEZE/THAW CYCLES. Recombinant, bovine prion protein (amino acids 25-244) fused to a His•Tag® sequence and expressed in E. coli. The cellular isoform of the prion protein (PrPC) is a glycosylphosphatidylinositol-anchored glycoprotein, normally expressed in neural and non-neural tissues, including skeletal muscle. In transmissible spongiform encephalopathies (TSE) or prion diseases, PrPC, which is soluble in non-denaturing detergent and sensitive to proteinase K (PK) treatment, represents the molecular substrate for the production of a detergent-insoluble and PK-resistant isoform, called PrPSc (prion scrapie). The interaction of PrPSc with PrPC causes the abnormal structural conversion of the latter resulting in TSE. Purity: ≥95% by SDS-PAGE. Ref.: Martins, V.R., et al. 2001. Braz. J. Med. Biol. Res. 34, 585. Zanusso, G., et al. 2001. Brain Pathol. 11, 182. Pruisner, S.B. 1998. Proc. Natl. Acad. Sci. USA 95, 13363. Pan, K.M., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 10962. Basler, K., et al. 1986. Cell 46, 417. |
